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The Sigma Users Group Galleries | all galleries >> User Galleries >> Users Galleries By Camera >> SD9 >> Steve Wolniak > Buccal epithelial cell (human - mine) 63X plan APO- 1.4x teleconverter
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27-AUG-2003

Buccal epithelial cell (human - mine) 63X plan APO- 1.4x teleconverter

The buccal (cheek) epithelial cell is a standard specimen for light microscopy. These cells are readily obtained, by scraping the inner surface of one's cheek and putting the cells onto a glass microscope slide. The cells here are unstained, but viewed with differential interference contrast (DIC) optics. As before, the SD-9 is attached to the microscope via a T-mount adapter, which I fabricated, and a 1.4 x Sigma Teleconverter is installed between the T-mount adapter and the camera body. The thin depth of field allows for optical sectioning of the cell - this image is focused on the upper surface of the same cell, where ridges in the surface are readily visible. The companion image is focused so that the nucleus is clearly visible.

This image was only manipulated in Sigma Photo Pro, and adjusted for brightness and contrast. Sharpness was reduced in the program to -0.5.

Sigma SD9
1/2s f/4.0 iso100 hide exif
Full EXIF Info
Date/Time27-Aug-2003 10:38:51
MakeSigma
ModelSD9
Flash UsedNo
Focal Length
Exposure Time0.50 sec
Aperturef/4
ISO Equivalent100
Exposure Bias
White Balance (-1)
Metering Modematrix (5)
JPEG Quality (6)
Exposure Programshutter priority (4)
Focus Distance

other sizes: small medium large original auto
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