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Peng Liang | profile | all galleries >> special_events >> cloning_lacz_gene tree view | thumbnails | slideshow
Harpeth Hall Girls Doing Research in my lab at Vanderbilt

They PCR amplified the lacZ gene encoding the beta-galactosidase
Saw the amplified DNA using agarose gel electrophoresis
cloned it into PCR-TRAP plasmid vector
Transformed the chimeric plasmid into E. coli
and got Blue colonies using X-gal as an indicater for beta-gal activity
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Peng Liang12-Jun-2006 04:28
Thanks Tingting!
My lab did not have that many white lab coats ( I meant clean ones) and thought these disposable yellow gowns for animal work would do the job. They thought these are the new styles! :) . They did tour our animal facility but were too scared to see the Saced mice!
Guest 11-Jun-2006 22:13
Nice pictures! And same pipets as mine.
Do they have to wear yellow gown all the time including doing cloning?
I only wear while sacrificing mices. =)

Tingting
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