The Seven Mile Bridge, is a famous bridge in the Florida Keys, in Monroe County, Florida, United States. It runs over a channel between the Gulf of Mexico and the Florida Strait, connecting Knight's Key (part of the city of Marathon, Florida) in the Middle Keys to Little Duck Key in the Lower Keys. Among the longest bridges in existence when it was built, it is one of the many bridges on US 1 in the Keys, where the road is called the Overseas Highway.

There are two bridges in this location. The older bridge, originally known as the Knights Key-Pigeon Key-Moser Channel-Pacet Channel Bridge, was constructed from 1909-1912 under the direction of Henry Flagler as part of the Florida East Coast Railway's Key West Extension, also known as the Overseas Railroad.

The spectacular bridge has attracted many film makers. Its film 'credits' include scenes in True Lies, 2 Fast 2 Furious, the James Bond film Licence to Kill, CrissCross, and Up Close & Personal. In True Lies, filmed in the early 1990s, a section of the old bridge is shown being destroyed by missile strikes. The missiles were edited in, and the explosions were done on an 80-foot (24 m) model of the bridge, but filming was done on the actual bridge, and the "destroyed" section is the former swing span, which had been removed upon completion of the new bridge.