The continuing advancement in cellular biology,sequencing techniques and cancer genomics analysis approaches, is making making cancer treatments more precise and effective. With single-cell sequencing & spatial transcriptomics along with gene editing techniques such as CRISPR/Cas9 have been used to study cancer stem cells in human tumors. The molecular signatures of cancer stem cells & cancer stem cells-initiated tumor cell lineage include gene mutation, protein expression, microRNA regulations,gene splicing, DNA methylation, and histone modification. These high resolution techniques offer doctors a unique opportunity for designing targeted cell immune-based therapies and Stem Cell Transplants in Cancer Treatment Stem cell transplants in Cancer patients are most often used to help people with leukemia or lymphoma. They may also be used for neuroblastoma and multiple myeloma.