During the Swedish Reformation in 1527, the Abbey was banned from accepting novices and its assets was confiscated by the crown in accordance with the Reduction of Gustav I of Sweden. The members were free to leave or to remain supported by an allowance from the estates formerly belonging to the Abbey. In 1566, these rights were confirmed, at which point the nuns were apparently still managing their school for daughters of the nobility. In 1577, only two nuns remained, and the last allowance to a nun of the Sko Abbey is recorded from 1588. After the Reformation, the monastery seems to have functioned as a girls' school and as a retirement home for older women.