I'm new at this, (Nikon D7000) but why would you use ISO 400 when you could drop the shutter speed to 1/100 at ISO 100 (especially at 16mm)...or, is the noise at ISO 400 imperceptable?
Also, from my experience with film, I would've stopped the lense down - or is this shot to demonstrate the performance of this lens (which I have) even at medium aperture?